Analysis on Health Resource Allocation and Service Utilization of Maternal and Child Health Care Institutions in Guizhou / 中国卫生经济

Objective:To discuss on the allocation and service utilization of health resources in maternal and child health care institutions and the existing problems in Guizhou Province,to provide a scientific basis for the further development of maternal and child health care.Methods:It analyzed the situation of health resource allocation,service utilization efficiency and maternal and child mortality in Guizhou province by means of statistical description,comparative analysis and correlation analysis.Results:The average number of health workers per hospital increased rapidly,the average number of beds per hospital grew slowly.The number of outpatient visits,the number of admissions and the number of people discharged were positive growth.The number of bed turnover,the working day of the bed,the bed use rate and the average hospitalization date appeared a certain degree of negative growth.Infant mortality rates,child mortality rates and maternal mortality rates under the age of five were all higher than the national average.Conclusion:Health resources of maternal and child health institutions in Guizhou should be tilted to the grassroots health institutions.It needed to further improve the efficiency of maternal and child health care use,strengthen the training of personnel and enhance the comprehensive service capacity of grassroots organizations.

Ginsenoside Rh₂ induces apoptosis and autophagy of K562 cells by activating p38 / 中国中药杂志

To study the effect of ginseng saponin Rh₂ in inducing apoptosis of human leukemia K562 cells, and explore its mechanism from the aspect of autophagy pathway. CCK-8 assay was used to examine the growth inhibition of human leukemia cell lines K562 treated with ginsenoside Rh₂; flow cytometry (FCM) was used to detect cell apoptosis; Hoechst staining was used to observe the changes of cell morphological apoptosis; Acridine and MDC staining were used to detect the effects of the Rh₂ on autophagy; Western blot and RT-PCR were used to detect the expression levels of the proteins closely associated with autophagy and apoptosis. In order to study the effect of autophagy in proliferation and apoptosis, we used the autophagy inhibitor (3-MA).CCK-8 indicated that Rh₂ at low concentration could effectively inhibit the proliferation of leukemia cellsin dose- and time-dependent manners in K562 cells; FCM indicated that Rh₂ induced apoptosis; Hoechest staining showed that K562 cells had typical apoptotic morphological changes by treated Rh₂; Acridine and MDC staining showed that Rh₂ enhanced the green fluorescence and a large number of acidic autophagy vesicles were present; Western blot and RT-PCR results showed that Rh₂ increased the expression levels of Beclin-1, LC3A, LC3B, activated Caspase-3 and p-p38 in K562 cells; application of autophagy inhibitors(3-MA) could weaken the inhibition effect of Rh₂ on proliferation and induction effect on apoptosis in K562 cells. Ginsenoside Rh₂ inhibited the proliferation and induced apoptosis probably through activating p-p38, and inducing cell autophagy signaling pathway in K562 cells.

Characterization of a novel impurity in bulk drug of lisinopril by multidimensional NMR technique / 浙江大学学报（英文版）（B辑：生物医学和生物技术）

During the routine impurity profile of lisinopril bulk drug by HPLC (high-performance liquid chromatography), a potential impurity was detected. Using multidimensional NMR (nuclear magnetic resonance) technique, the trace-level impurity was unambiguously identified to be 2-(-2-oxo-azocan-3-ylamino)-4-phenyl-butyric acid after isolation from lisinopril bulk drug by semi-preparative HPLC. Formation of the impurity was also discussed. To our knowledge, this is a novel impurity and not reported elsewhere.